Fig. 4: Scavenging ROS with Cu5.4O USNPs in vitro. | Nature Communications

Fig. 4: Scavenging ROS with Cu5.4O USNPs in vitro.

From: Ultrasmall copper-based nanoparticles for reactive oxygen species scavenging and alleviation of inflammation related diseases

Fig. 4: Scavenging ROS with Cu5.4O USNPs in vitro.

a Representative ROS staining (green fluorescence) of HEK293 cells under different treatment conditions. b ROS levels in untreated and Cu5.4O USNPs-treated HEK293 cells incubated with 250 μM H2O2. c Statistical analysis of ROS levels in HEK293 cells under different treatment conditions. d In vitro cell viabilities of HEK293 cells under different treatment conditions. e FACS results of cell apoptosis and necrosis distribution in untreated and Cu5.4O USNPs-treated HEK293 cells. f Statistical analysis of necrotic and apoptotic cell ratios in HEK293 cells under different treatment conditions. In c, d and f, data represent means ± s.d. from four (c, f) or six (d) independent replicates. ***P < 0.001; n.s., no significance, one-way ANOVA. Source data are provided as a Source Data file.

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