Fig. 1: Workflow and results for identification of proteins enriched at evolutionary conserved RNA folds.
From: The RNA fold interactome of evolutionary conserved RNA structures in S. cerevisiae
a Schematic of the SILAC-based quantitative RNA–protein interactomics workflow. b Conservation analysis of the conserved fold in the PMA1 mRNA by RNAz. Dots indicate unpaired bases and brackets paired bases. Gray bars represent sequence conservation among the indicated 5 yeast species. Folded structure shown on the right, with positional entropy values ranging from red/yellow (lower entropy) to green/blue (higher entropy). c Two-dimensional interaction plot comparing the interactors for PMA1 wild-type hairpin with the mutated fold. d Heatmap showing enrichment values for the 162 protein-binding partners to the 186 investigated RNA folds. e Venn diagram showing overlap of interactors according to genomic position of the RNA fold (5′UTR, CDS, 3′UTR). f Dot-plot displaying the number of binders identified to each investigated RNA fold grouped by localization within the mRNA (13 5′UTR, 136 CDS and 37 3′UTR RNA folds) (Supplementary Data 2).
