Fig. 2: The CRISPRi-based toggle switch (TS).
From: Multistable and dynamic CRISPRi-based synthetic circuits
a Topology of the bistable TS and the monostable controls. b Molecular implementation. Symbols as described in Fig. 1. c Behavior of TS and control circuits over time in response to two induction schemes: Ara-nothing-AHL-nothing (left) or AHL-nothing-Ara-nothing (right). The percentage of green cells was determined by flow cytometry for four biological replicates (shown are the mean and the s.d.). d Histograms showing representative distributions of the TS cells according to their green fluorescence for the four timepoints indicated in panel c: (i) after Ara induction, (ii) after Ara followed by no inducer, (iii) after Ara-nothing-AHL, and (iv) after Ara-nothing-AHL-nothing. Black: cells below threshold for ‘HIGH’ state (gray line); green: cells in ‘HIGH’ state. Source data are provided as a Source Data file.
