Fig. 5: Transmission of recombinant hαSyn into Reporter-cells. | Nature Communications

Fig. 5: Transmission of recombinant hαSyn into Reporter-cells.

From: A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol

Fig. 5: Transmission of recombinant hαSyn into Reporter-cells.

a Equally scaled representative epifluorescence images of Reporter-cells after incubation for 14 h with recombinant hαSyn monomers added to the culture medium. Monomers were either administered alone (hαSyn) or pre-mixed with the commercial cationic lipid mixture RNAiMAX (hαSyn + iMAX). hβSyn + iMAX was used as negative control. Scale bar represents 20 µm. b Quantification of the percentage of EGFP-positive Reporter-cells. Error bars represent the SEM from three independent experiments (n = 3). Per replication and condition >2300 cells were analyzed. One-way ANOVA with multiple comparison Tukey's post hoc test **p < 0.01, ns: not significant. Source data is available as a Source data file.

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