Fig. 5: Structures of Lpg2149 in complex with MavC and MvcA. | Nature Communications

Fig. 5: Structures of Lpg2149 in complex with MavC and MvcA.

From: Insights into catalysis and regulation of non-canonical ubiquitination and deubiquitination by bacterial deamidase effectors

Fig. 5: Structures of Lpg2149 in complex with MavC and MvcA.

a, b Overall structures of the Lpg2149+MavC (a) and Lpg2149+MvcA (b) complexes. Lpg2149 was shown in orange. MavC and MvcA has the same color code as in Fig. 1a. c, d Detailed hydrogen bonding (c) and hydrophobic (d) interactions between Lpg2149 and MavC, with the same color code as in a. e Structural superimposition between MavC+Lpg2149 and MavC+Ub complexes by using MavC (gray) as the reference point. Lpg2149 and Ub were shown in orange and black, respectively. f Lpg2149 can inhibit the Ub deamidation activity of both MavC and MvcA. g Lpg2149 can inhibit the UBE2N ubiquitination activity of MavC. h Lpg2149 can inhibit the UBE2N~Ub deubiquitination activity of both MavC and MvcA. Source data are provided as a Source Data file. Experiments in f–h were repeated independently three times with similar results.

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