Fig. 1: Traceless lipid-facilitated acceleration of NCL.
From: Traceless native chemical ligation of lipid-modified peptide surfactants by mixed micelle formation
a Schematic representation of traceless lipid-facilitated NCL via micelle mixing. b NCL reaction between aliphatic alkyl peptide thioesters (1a–c, 5a or 6a) and photocaged cysteine-based peptides (2a,b), which yields the desired polypeptides (3a,b, 7 and 8, respectively). Control reactions were performed with peptides containing short alkyl chains (1d, 2c, 5b, and 6b). Subsequent photocleavage reaction of the ligated products (3a,b, 7 or 8) leads to the formation of the uncaged polypeptides (4, 9, and 10, respectively).
