Fig. 3: Various ligations (Asn-Cys, Leu-Cys) and photocleavage reaction.
From: Traceless native chemical ligation of lipid-modified peptide surfactants by mixed micelle formation
a Kinetic measurements of NCL between the peptide thioesters LYRMN-αCOSC8 (5a) (1 mM) or LYRML-αCOSC8 (6a) (1 mM) and the peptide CDap(PhC16)ANK (2b) (1 mM). Decapeptide (7 and 8, respectively) formation was monitored over time using combined HPLC-ELSD-MS measurements. Error bars represent standard deviations (SD) (n = 3). b Photocleavage reaction of the auxiliary on NCL product LYRMGCDap(PhC16)ANK (3b) to generate the decapeptide LYRMGCDapANK (4). The photocleavage reaction was monitored using HPLC-ELSD-MS (raw ELSD data shown).
