Fig. 5: Derivatization of the natural protein Ubiquitin by lipid-facilitated NCL.
From: Traceless native chemical ligation of lipid-modified peptide surfactants by mixed micelle formation
a HPLC (210 nm) traces corresponding to the precursors Ubi-αCOSC8 (16) and CK(PhC16)ANK (S4) (top) and the ligated product Ubi-CK(PhC16)ANK 17 (after 5 h of NCL reaction; bottom). Retention times were verified by MS. b ESI-TOF MS spectrum of the ligated product 17. Deconvoluted spectrum calculated from this ESI-TOF spectrum is also shown in the top left. The deconvolution value (9485.0) corresponds with the molecular weight of the product.
