Fig. 3: Expression of α-globin is gradually upregulated during in vivo erythroid differentiation.

a Scheme of erythroid differentiation showing the various populations analyzed. b Example FACS plot showing the gating strategy used to isolate erythroid progenitors from mouse fetal liver. c Expression (in counts per million) of α-globin transcripts in each population as determined by single-cell RNA-seq. Mean expression for each population is given at the top of each bar. d Representative RNA-FISH images showing detection of nascent α-globin transcripts in sorted early erythroid progenitors. Scale bar is 3 μM for each image. e RNA-FISH quantification, showing the mean ± s.d. of n = 3 independent experiments (except for brain and “no primary” negative controls, which have n = 2). P-values were calculated by two-tailed paired t-tests (S0-low vs S0-medium P = 0.041, S0-low vs S1 P = 0.002). Source data are provided as a Source Data file.