Fig. 4: Upregulation of α-globin expression correlates with increased chromatin accessibility and enhancer-promoter interactions.
From: Dynamics of the 4D genome during in vivo lineage specification and differentiation
Tiled-C contact matrices of 500 kb spanning the mouse α-globin locus in sequential stages of in vivo erythroid differentiation at 2 kb resolution. Contact frequencies represent normalized, unique interactions in two replicates. The black and gray bar below each matrix represent the pre-existing TAD (chr11:32,080,000-32,245,000) and erythroid-specific sub-TAD (chr11:32,136,000-32,202,000), respectively. Matched open chromatin (ATAC) profiles are shown underneath the matrices and represent normalized data from 3 S0-low, S0-medium, and S1 replicates and 2 S2 and S3 replicates. The ATAC profiles are shown at different scales to highlight changes in accessibility in early stages of differentiation. Gene annotation (α-globin genes highlighted in red), open chromatin (ATAC; α-globin enhancers highlighted in red), and CTCF occupancy in mature mouse erythroblast cells are shown at the top. Coordinates (mm9): chr11:31,900,000–32,400,000.
