Fig. 1: Induction of Blimp1 protein expression during pregnancy and PR-Cre-driven deletion in decidual tissues.
From: The transcriptional repressor Blimp1/PRDM1 regulates the maternal decidual response in mice
a Blimp1 IHC in virgin, E3.5, E4.5, E5.5 and E6.5 wild-type uteri. In virgins, Blimp1 was detected in a small number of cells underlying the luminal epithelium. Upon fertilisation, Blimp1 was induced in uterine stroma (E3.5) followed by strong upregulation at the site of implantation in both the decidualising stroma and luminal epithelium (E4.5-E6.5). Results are representative of triplicate staining experiments performed using independent samples. b Western blot analysis confirmed strong induction of Blimp1 protein in the uterus during pregnancy. Source data are provided as a Source Data file. Duplicate experiments were performed with comparable results. c IHC confirms loss of Blimp1 protein in decidual stromal cells in Blimp1 mutant mice at E6.5. Data from 5 tissue sections from triplicate decidual samples per genotype. Source data are provided as a Source Data file. Bars represent mean. Two-tailed unpaired Student’s t-test ***p = 1.36 × 10−4. LE = luminal epithelium, GE = glandular epithelium, B = blastocyst, E = embryo, ExE = extra-embryonic ectoderm, Ep = epiblast, VE = visceral endoderm, M = mesometrial, AM = antimesometrial. Scale bar = 100 μm.
