Fig. 5: Detection of hormone titers in susceptible and resistant P. xylostella. | Nature Communications

Fig. 5: Detection of hormone titers in susceptible and resistant P. xylostella.

From: MAPK-dependent hormonal signaling plasticity contributes to overcoming Bacillus thuringiensis toxin action in an insect host

Fig. 5: Detection of hormone titers in susceptible and resistant P. xylostella.

a Representative MRM chromatograms of the standard solutions of JHs (JH II and JH III, 5 ng), 20E (2 ng), methoprene (0.1 ng), and 22S, 23S-homobrassinolide (0.1 ng) detected by UPLC–MS/MS. b Hormone titers in whole developmental stages of the DBM1Ac-S strain. EG: eggs; L1-4: first- to fourth-instar larvae; P: pupae; A: adults. Samples were collected daily in each stage for hormone titer detection. JH II (c) and 20E (d) titers from third- to fourth-instar larvae of both DBM1Ac-S and NIL-R strains. Samples were collected every 12 h in both stages for accurate detection of hormone titer changes. e, f Box plots show JH II (e) and 20E (f) titers in all of the susceptible and resistant P. xylostella strains. Each orange dot represents mean hormone titer in two 1-day-old fourth-instar larvae and the black plus sign indicates mean hormone titer of all the detected individuals in each strain. Median and quartile values are provided by the central line and box boundaries, whiskers show min to max values. Data are presented as mean values ± SEM, n = 3 (b–d) and n = 10 (e, f) biologically independent samples, *p < 0.05, ***p < 0.001, one-way ANOVA with Holm–Sidak’s test was used in (e) and (f) for comparison. Source data are provided as a Source Data file.

Back to article page