Fig. 4: The ovarian somatic depletion of MEP-1 or Mi-2 results in TE and TE reporter derepression in vivo. | Nature Communications

Fig. 4: The ovarian somatic depletion of MEP-1 or Mi-2 results in TE and TE reporter derepression in vivo.

From: The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation

Fig. 4

a X-Gal staining of egg chambers that contain the somatic TE reporter (Gypsy-lacZ) in which the indicated genes were knocked down by constitutive (left panel) or conditional (right panel) somatic RNAi. Conditional somatic knockdown was restricted to adult flies. The control was a shRNA against the white gene (shwhite). b, c Fold change (log2) in the steady-state RNA levels of the Gypsy-lacZ reporter (lacZ) and of three endogenous TEs in ovaries after constitutive (tj-GAL4 > sh) (b) or conditional (t-jGAL4;tubGAL80ts > sh) (c) somatic knockdown, using the indicated RNAi. RNA levels were quantified relative to RpL32 levels and normalized to control knockdown (shwhite). Lines represent means ± SD for n = 3 biologically independent samples. Source data for b, c: Supplementary Data set 4.

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