Fig. 6: MEP-1 interacts with Su(var)2-10 and induces transcriptional silencing upon recruitment to DNA.

a Western blot analysis of co-IP of (left panel): MEP-1 in OSC nuclear extract expressing either FLAG-FFL-Su(var)2-10 or FLAG-FFL-mCherry as a negative control or of (right panel): GFP-Trap in OSC nuclear extract expressing either Su(var)2-10-GFP or GFP alone. b Graph shows the normalized co-IP efficiency of several preys (MEP-1, MEP-1ΔCt, MEP-1ΔNt, Mi-2, and the negative mCherry control) with the FLAG-FFL-Su(var)2-10 bait. Dots show values of n = 4 biologically independent samples, lines represent mean ± SEM. c Targeting MEP-1 to reporter DNA induces silencing in vivo. Schematic representation of the 8xlacO-GFP reporter. The lower panels show the GFP fluorescence signal of LacO-GFP reporter in egg chambers that express the LacI (left) or LacI-MEP-1 fusion proteins (right) under the control of the germline-specific nos-GAL4 driver. Scale bars, 10 μm. d Dots showing quantification of eGFP RNA levels in ovaries that express LacI or LacI-MEP-1 under the control of the germline-specific nos-GAL4 driver. RNA levels are relative to RpL32 level. Mean values ± SEM of n = 3 biologically independent samples are represented. e Comparison of H3K9me3 and H3K9ace amounts on the LacO-GFP reporter, expressed in percentage of immunoprecipitated H3, in ovaries that express LacI or LacI-MEP-1 fusion proteins. P-values were calculated using sample data that displayed normal distribution (tested with the Shapiro–Wilkinson test). Variance homogeneity was tested with the Levene’s test and then the two-tailed Student’s t-test was used. ***P-value < 0.05. Mean values ± SEM of n = 3 biologically independent samples are represented. Source data for b, d, e: Supplementary Data set 6 and Supplementary Table 2 for e; uncropped blot images are provided in Supplementary Data set 1.