Fig. 7: Summary of synergistic gene editing effects favoring HDR outcomes.
From: Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
Synchronization and release of cells into the HDR permissive S and G2 phases of the cell-cycle synergizes with DNA repair inhibition to improve HDR rates. Additional non-cell-cycle-dependent effects of cold shock may also play a role. Leveraging these mechanisms, homozygous mutations are generated at high efficiency, while heterozygous mutations may be generated without indels by using a combination of mutant and blocking ssODN templates.
