Fig. 4: Mutations and segregation in VviINP1.

a Alignment of the first 100 bp of 20 VviINP1 coding sequences representing 12 F, 5 H and 3 M haplotypes along with two M. rotundifolia INP1 coding sequences from F and M haplotypes. Alignment revealed an F-linked 8 bp INDEL in VviINP1 throughout Vitis and shared with M. rotundifolia. Yellow, purple and gray colors represent F, M and H haplotypes, respectively. b Phylogenetic subtree of the INP1 coding sequences from Vitis spp. and M. rotundifolia. The tree was rooted with INP1 sequences from seven outgroups (see “Methods”). Tree branches are colored according to sex-determining region haplotype. Scale bar is in the unit of the number of substitutions per site. Sequence length in bp is indicated in parentheses. c Marker assay amplifying INP1 fragment without (top panel, 609 bp) or with (bottom panel, 605 bp) the 8 bp deletion. Actin was used as a PCR positive control (99 bp fragment). This assay was performed three times. Vvs Vv sylvestris, L Ladder, C− negative control, Vv vinifera: Bs seeded Black Corinth, BC seedlees Black Corinth, CF Cabernet Franc, CS Cabernet Sauvignon, Ca Carménère, Ch Chardonnay, GB Gouais blanc, Ri Riesling, SB Sauvignon blanc, Se Semillon, Zi Zinfandel, Vv sylvestris: 1, DVIT3351.27; 2, DVIT3603.07; 3, DVIT3603.16; 4, O34-16; Va V. arizonica, Vp V. piasezkii, Vr V. romanetii, Mr M. rotundifolia. Source data are provided as a Source Data file.