Fig. 6: Peptide R50 organizes into amyloid structures and interferes with ZIKV replication.
a Hydrodynamic radius (RH) calculated from the regularization fit of DLS data of peptide R50 (10 µM) maturation over time. b Th-T emission signal of 10 µM peptide R50 at 485 nm after excitation at 440 nm as a function of time (n = 3 independent experiments). c TEM image of 10 µM peptide R50 after 6 h incubation. d Soluble fraction of peptide R50 (10 µM) determined after ultracentrifugation (250,000 × g for 30 min), measured over time, and mean ± SD is shown (n = 3 independent experiments). Dose-dependent effect of 7-DMA (e), peptide R50 (f), and peptide 12B (g) on the fraction of ZIKV-infected cells following 48 h of infection with a MOI of 0.1. Data are normalized to DMSO-treated, infected cells (100% infected cells) and mean values ± SD are shown (n = 3 independent experiments). Representative images are shown in Supplementary Fig. 10. Dose-dependent effect of 7-DMA (h), peptide R50 (i), and peptide 12B (j) on cell viability of Vero E6 cells without ZIKV infection. Data are normalized to DMSO-treated, noninfected cells (100% cell viability) and the mean values ± SD are shown (n = 3 independent experiments).
