Table 1 Oligo DNA substrates used in this study.
From: Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51
Name | Sequence (5′ to 3′) |
|---|---|
16A(−) | AAATGAACATAAAGTAAATAAGTATAAGGATAATACAAAATAAGTAAATGAATAAACATAGAAAATAAAG TAAAGGATAT AAA |
[FAM]16A(−) | [FAM]AAATGAACATAAAGTAAATAAGTATAAGGATAATACAAAATAAGTAAATGAATAAACATAGAAAATAAAG TAAAGGATAT AAA |
16A(−)_3 × 2AP | AAATG[2AP]ACATAAAGTAAAT[2AP]AGTATAAGGATAAT[2AP]CAAAATAAGTAAATGAATAAACATAGAAAATAAAGTAAAGGATATAAA |
16A(−)_40 bp | AAATGAACATAAAGTAAATAAGTATAAGGATAATACAAAA |
[FAM]16A(−)_40 bp | [FAM]AAATGAACATAAAGTAAATAAGTATAAGGATAATACAAAA |
16A(+)_40bp | TTTTGTATTA TCCTTATACT TATTTACTTT ATGTTCATTT |
16A(+)[ROX]_40 bp | TTTTGTATTA TCCTTATACT TATTTACTTT ATGTTCATTT-[ROX] |
[TAMRA]dT72 | [TAMRA]TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT |
[TAMRA]16A(−)_72 bp | [TAMRA]AAATGAACATAAAGTAAATA AGTATAAGGATAATACAAAA TAAGTAAATGAATAAACATAGAAAATAAAGTA |
16A(+)_72 bp | TACTTTATTTTCTATGTTTATTCATTTACTTATTTTGTAT TATCCTTATACTTATTTACTTTATGTTCATTT |
16(A)(−)_F + R_73 bp | AAATGAACATAAAGTAAATAAGTATAAGGA[Fluorosein-dT]AATAC AAAAT[ROX-dT]AAGTAAATGAATAAACATAGAAAATAAAGTA |
16(A)(+)_73bp | TACTTTATTTTCTATGTTTATTCATTTACTTAATTTTGTATTATCCTTATACTTATTTACTTTATGTTCATTT |
