Fig. 3: Accumulation of LDs in ZIKV-infected placenta.

a Representative thin sections of matched mock and ZIKV-infected placentas 5 dpi. Immunostaining is performed with anti-NS3 (red) and anti-CK7 (yellow) antibodies. LDs are stained with the BODIPY 493/503 dye (green) and nuclei with DAPI (blue). High magnification of the indicated area (arrowhead) is shown in insets. Scale bar, 50 μm. Data are representative of at least three independent experiments. b Oil Red O staining of neutral lipid in tissue sections from matched mock- and ZIKV-infected placenta (red). Nuclei are counterstained with hematoxylin. High-magnification images of the indicated area (arrowhead) are shown in insets. Scale bar, 50 μm. c Quantification of Oil Red O staining by Image J showing increased staining area in the infected placental sections. All data are presented as mean ± SEM of first trimester placentas from five independent donors. P values are computed using paired two-tailed Student’s t test. Source data are provided as a Source Data file.