Fig. 2: Robustness of TFs with respect to stimulation duration and intensity.

a Ca²⁺ (top) and RBC velocity (bottom) responses to single sniff (120 ms), 1 s, 2 s, and 5 s odor stimulations. Using the standard μTF (optimized with ET 1%, 5 s), vascular response predictions (orange traces) are robust for all durations. b Increasing the odor concentration from 1% (top traces, average of two trials) to 6% ET (bottom traces, average of three trials) reveals a delayed secondary vascular response, which is not correctly predicted (orange trace) with the standard TF (1% ET, 5 s). c Vascular responses after subtraction of their prediction using the standard TF (n = 5 mice, data from b in black). Note that trace fluctuations between 5 and 15 s (gray background) result from slight differences of onset, slope, and response peak between real and predicted responses. The second vascular component is clearly delayed by ∼10 s. d Left, novel TFs optimized on vascular responses after subtraction (the thick trace is the TF from the data in b and c). Note the heterogeneity in peak jitter and shape. Right, combination of the two μTFs (standard μTF in orange, second component μTF in gray) can predict correctly the entire vascular response (see inset, same trace as in b) after adjustment of their corresponding amplitude.