Fig. 3: Co-registration of fUS (single voxel) and two-photon imaging.
From: Transfer functions linking neural calcium to single voxel functional ultrasound signal
a Schematics of the two imaging systems. The ultrasonic probe is attached to the ×20 microscope objective by means of a 3D-printed holding system. A 50-μm glass bead, embedded in agar, is first localized with 2P imaging. The ultrasonic probe is then translated over the bead and placed at a position where the fUS signal maxima in x, y, and z are centered in a given voxel. The fUS and 2P imaging systems can then be displaced back and forth to the same co-registered location with a micrometric resolution. b Intensity profiles of fUS signals in x, y, z. FWHM for full width at half maxima. Square and circle points were acquired during back and forth acquisitions. c ΔPD/PD fUS activation map of an olfactory bulb coronal section in response to 1%, 5 s ET. The enlarged area shows the voxel centered on the most responsive glomerulus (first imaged with 2P) plus its five neighboring voxels. d From bottom to top, Ca2+ and RBC velocity glomerular responses (2P imaging), ΔPD/PD fUS responses from the co-registered voxel and the six voxels.
