Fig. 3: DORIS mimics natural transcription to repeatedly access information.

a File A was separated using non-PCR-based magnetic separation while the database was recovered (Retained Database) (n = 3 for each condition). T7-based in vitro transcription was performed directly on the bead-immobilized file for up to 48 h to generate RNA. Reverse transcription converted the RNA to complementary DNA (cDNA) while the immobilized file A was released back into the database (Retained File) (n = 3 for each condition). b The amount of retained database (light shading) and retained file (dark shading) after file A was accessed by oligo A’ was measured by qPCR and plotted as a percentage of the original amount of each file that was in the database. The specificity of file access is evident by the absence of file B and C in the Retained File. The presence of T7 RNA polymerase (RNAP) did not affect the retention of file A. c File A was repeatedly accessed 5 times. The amounts of file A, B and C in the database were measured by qPCR and plotted as the amount of each file in the database after each run (n = 3 for each condition), normalized to the original amount of each file prior to the 1st access. Values represent the arithmetic mean. Error bars are s.d., n = the number of replicate file accesses. Source data are provided as a Source Data file.