Fig. 7: Altering metabolism by exogenous supplementation modifies mutational buffering capacity. | Nature Communications

Fig. 7: Altering metabolism by exogenous supplementation modifies mutational buffering capacity.

From: Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis

Fig. 7

a Expression of Wt, C1, and C6 GFP mutants was induced in CSH4 cells either growing in LB or in LB containing different metabolites. Average of log2 of fold-change in median of GFP/mCherry fluorescence as measured by single-cell fluorescence from three replicates is shown as heatmap. b Mean of fold-change in median of GFP/mCherry fluorescence in few metabolites (Alanine, Betaine, Proline, Aspartate) in CSH4 is shown as bar graph along with standard deviations from three biological replicates and P-value calculated with respect to Wt GFP by 2-sided Students’ t-test, *p-value C1 Alanine: 0.02, Betaine: 0.01, Proline: 0.01; C6 Betaine: 0.03. (Source data are provided as source data file Fig. 7).

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