Fig. 4: Analysis of mice hematopoiesis with Poincaré maps.

a Developmental hierarchy proposed by Moignard et al.¹ and Haghverdi et al.¹⁶. b Rotated Poincaré map with respect to reassigned root. Gray cluster represent a cluster of potential outliers or "mesodermal'' cells as suggested by Moignard et al.¹. Lineage slices were obtained with Poincaré maps (see Methods). c Composition of detected lineages in terms of the presence of cells from different developmental stages. d Violin plots of diffusion and Poincaré pseudotime for each stage of embryo developmentt. The ordering of cells proposed by Poincaré maps here has a much better agreement with developmental stages than ordering originally proposed by Haghverdi et al.¹⁶: we see a very clear correlation of Poincaré pseudotime with actual developmental time. e Gene expression of main hemogenic genes. Hemogenic genes of erythroid lineage are already expressed at the PS and NP stages.