Fig. 3: Rab11b is required for breast cancer brain metastasis.
From: Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth
a Normalized mean RAB11B expression in MDA-231 cells, relative to pLKO.1 empty vector. Three independent experiments. Bars, mean ± s.d. ANOVA, Dunnett’s multiple comparison. b Rab11b immunoblots for MDA-231 cells expressing indicated constructs. c Rab11b-GTP immunoblot for MDA-231 cells. d Normalized RAB11B expression in MDA-231 cells cultured alone or with primary murine glia for three days, relative to pLKO.1 alone. n = 3 independent experiments. Bars, mean ± s.d. Two-way ANOVA, Tukey’s multiple comparison. e Rab11b immunoblots for MDA-231 cells cultured alone or with primary murine glia for five days followed by removal of glial cells. f Representative H&E and IHC images for mice intracranially injected with MDA-231-tdTomato control or shRab11b cells. Scale bar 100 μm. g Representative H&E and IHC images for mice intracardially injected with MDA-231-Br-EGFP control or shRab11b cells. Scale bar 100 μm. h Quantitation of Ki-67 staining. n = 2 independent experiments. Bars, mean ± s.d. ANOVA, Dunnett’s multiple comparison. i Incidence of MDA-231-Br brain metastasis determined by visible GFP signal at 28 dpi. Analysis of contingency, Fisher’s exact test. For all panels, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
