Fig. 7: Statins decrease breast cancer brain metastasis and improve survival.
From: Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth
a–c MDA-231-Br-GFP cells were intracardially injected, and given daily intraperitoneal injections of vehicle or 1 mg/kg pitavastatin or 5 mg/kg simvastatin. a Incidence of brain metastasis determined by visible GFP signal at 28 dpi. Analysis of contingency, Fisher’s exact test. b Quantification of Ki-67 staining for proliferation. n = 2 independent experiments. Bars, mean ± s.d. ANOVA, Tukey’s multiple comparison. c Survival determined by daily monitoring for the apearance of neurological symptoms or euthanasia criteria. Log rank test. d–g MDA-231-tdTomato cells were intracranially injected, and given daily intraperitoneal injections of vehicle or 1 mg/kg pitavastatin or 5 mg/kg simvastatin. d Representative images of brain metastasis at 34 dpi. e Quantification of Ki-67 staining for proliferation. n = 2 independent experiments. Bars, mean ± s.d. ANOVA, Tukey’s multiple comparison. f Scoring of ITGB1 immunostaining. n = 2 independent experiments. Analysis of contingency, Fisher’s exact test. g Representative images of Rab11b and ITGB1 immunostaining. Scale bar 100 μm. For all panels, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
