Fig. 2: Structure of HKU2 NTD and comparisons. | Nature Communications

Fig. 2: Structure of HKU2 NTD and comparisons.

From: Cryo-EM structures of HKU2 and SADS-CoV spike glycoproteins provide insights into coronavirus evolution

Fig. 2: Structure of HKU2 NTD and comparisons.

a Structure of HKU2 NTD. Side views of HKU2 NTD are shown in two orthogonal directions. Disulfide bonds are shown as red sticks. Conserved disulfide bonds are labeled blue; other disulfide bonds are labeled black. Top sheet, middle sheet, bottom sheet, and bottom helix are partitioned by gray dashed lines. Comparison of HKU2 NTD and galectin3 are shown in the right panel. HKU2 NTD is colored salmon; galectin3 is colored green. PDB code: galectin3, 1A3K. b Subtype I NTD of α-coronavirus. Structural alignments of HKU2 NTD with HCoV-NL63 NTD1, and with HCoV-NL63 NTD2 are shown. HKU2 NTD is colored salmon. HCoV-NL63 NTD1 and NTD2 are colored gray. Disulfide bonds are shown as red sticks. Disulfide bonds conserved in both types of NTDs are indicated by red arrows; disulfide bonds conserved in subtype I NTD are indicated by blue arrows; PDB code: HCoV-NL63, 5SZS. c Subtype II NTD of α-coronavirus. Structural alignments of HCoV-229E NTD with HCoV-NL63 NTD1, and with HCoV-NL63 NTD2 are shown. HCoV-229E NTD is colored marine. HCoV-NL63 NTD1 and NTD2 are colored gray. Disulfide bonds conserved in both types of NTDs are indicated by red arrows; disulfide bonds conserved in subtype II NTD are indicated by orange arrows. PDB codes: HCoV-229E, 6U7H. d β-coronavirus NTDs resemble subtype I. Structural alignments of HKU2 NTD with SARS-CoV NTD, and with MHV NTD are shown. Disulfide bonds are shown and labeled the same as in b. Ceilings in β-coronavirus NTDs are shown in yellow. PDB codes: SARS, 5XLR; MHV, 3JCL. e γ-coronavirus IBV NTD resembles subtype I. Structural alignment of HKU2 NTD with IBV NTD is shown. Disulfide bonds are shown and labeled the same as in b. The additional loops in IBV NTD is shown in yellow. PDB code: IBV, 6CV0. f δ-coronavirus PdCoV NTD resembles subtype II. Structural alignment of HCoV-229E NTD with PdCoV NTD is shown. Disulfide bonds are shown and labeled the same as in c. The additional helices in PdCoV NTD are shown in yellow. PDB codes: HCoV-229E, 6U7H; PdCoV, 6B7N.

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