Fig. 5: Cold exposure does not induce glucose uptake or beiging in BMAT.

Cold-induced glucose uptake was assessed by PET/CT, as described in Supplementary Fig. 3A. a PET/CT images representative of 7 control, 7 acute and 8 chronic cold mice show increased ¹⁸F-FDG uptake in interscapular and paraspinal BAT but not in tibiae; some ¹⁸F-FDG signal is evident in skeletal muscle of each group. b, c ¹⁸F-FDG uptake in the indicated tissues was determined by PMOD analysis of PET/CT scans (b) or gamma counting (c). d Representative micrographs of H&E-stained tissues, showing that cold exposure decreases lipid content in BAT and promotes beiging of iWAT, but these effects do not occur in BMAT; scale bar = 150 µm. e–g Effects of cold exposure on expression of transcripts relating to brown and beige adipocyte function (Ucp1 and Dio2) and fatty acid oxidation (Cpt1b and Ppara) in BAT, iWAT and whole bones. ND not detectable. Data in b, c, e–g are shown as mean ± SEM of the following numbers of mice per group: Control, n = 7 (b, c, e, g) or 6 (f); acute cold, n = 7 (b, e), 8 (c, g) or 6 (f); chronic cold, n = 8 (b, c, g) or 7 (e, f). Within each tissue, significant differences between groups are indicated by ^#P < 0.01, *P < 0.05, **P < 0.01 or ***P < 0.001. The following groups of data are non-normally distributed and were assessed using the Kruskal–Wallis test with Dunn’s test for multiple comparisons: b Heart, Sk. Muscle and Femur bone; c iWAT and gWAT; e Dio2; f Dio2 and Cpt1b; g Dio2 and Ppara. Data for all other tissues (b, c) or transcripts (e–g) are normally distributed and were assessed using one-way ANOVA with Dunnet’s or Tukey’s tests for multiple comparisons. Source data are provided as a Source Data file. See also Supplementary Figs. 3–5.