Table 2 Sequences of primers used for qPCR.
From: Bone marrow adipose tissue is a unique adipose subtype with distinct roles in glucose homeostasis
Species | Transcript | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|---|
H. sapiens | ADIPOQ | TCCTCACTTCCATTCTGACTGC | GTAGAACAGCTCCCAGCAACA |
H. sapiens | CPT1B | CTGGTGCTCAAGTCATGGTG | CTGCCTGCACGTCTGTATTC |
H. sapiens | INSR | TAGACGTCCCGTCAAATATTGC | GAAGAAGCGTAAAGCGGTCC |
H. sapiens | IPO8 | TTTCCCCTCAAATGTGGCAGC | CTTCTCCTGCATCTCCACATAGT |
H. sapiens | IRS1 | AGAGGACCGTCAGTAGCTCA | TCTCTCATGACACGGTGGTG |
H. sapiens | IRS2 | CACCTACGCCAGCATTGACT | GAAACAGTGCTGAGCGTCTTC |
H. sapiens | METRNL | CCACAGGCTTCCAGTACGAG | TCAGGCTCGTGGGTAACTTG |
H. sapiens | PPARG | TCATGCTTGTGAAGGATGCAAG | ATCCCCACTGCAAGGCATTT |
H. sapiens | PRDM16 | GAGGAGAGAGATTCCGCGAG | CCCGGTTGGGCTCATACAT |
H. sapiens | RNA18SN5 | CGATGCTCTTAGCTGAGTGT | GGTCCAAGAATTTCACCTCT |
H. sapiens | SLC2A1 | TCCCTGCAGTTTGGCTACAA | CAGGATGCTCTCCCCATAGC |
H. sapiens | SLC2A3 | TAGATTACAGCGATGGGGACAC | GTAGCCAAATTGGAAAGAGCCG |
H. sapiens | SLC2A4 | TCGGGCTTCCAACAGATAGG | GTTGTACCCAAACTGCAGGG |
H. sapiens | TGM2 | GGAGTATGTCCTCACCCAGC | CGTTCTTCAGGAACTTGGGGT |
H. sapiens | UCP1 | GTGTGCCCAACTGTGCAATG | ACGTTCCAGGATCCAAGTCG |
M. musculus | Cpt1b | TGTCTACCTCCGAAGCAGGA | CGGCTTGATCTCTTCACGGT |
M. musculus | Dio2 | TCTTCCTGGCGCTCTATGAC | ACCACACTGGAATTGGGAGC |
M. musculus | Metrnl | CCTGGAGCAGGGAGGCTTAT | TCGGACAACAAAGTCACTGGT |
M. musculus | Ppara | CCTGAACATCGAGTGTCGAATAT | TCTTCTTCTGAATCTTGCAGCT |
M. musculus | Pparg | GGAAAGACAACGGACAAATCAC | TACGGATCGAAACTGGCAC |
M. musculus | Ppia | CACCGTGTTCTTCGACATCA | CAGTGCTCAGAGCTCGAAAGT |
M. musculus | Prkaa1 | ACCAGGTCATCAGTACACCATC | ACACCGGAAAGGATCTGCTG |
M. musculus | Rn18s | CGATGCTCTTAGCTGAGTGT | GGTCCAAGAATTTCACCTCT |
M. musculus | Slc2a4 | ACTCATTCTTGGACGGTTCCTC | CACCCCGAAGATGAGTGGG |
