Table 3 Antibodies used for Western Blotting.
From: Bone marrow adipose tissue is a unique adipose subtype with distinct roles in glucose homeostasis
Protein | Antibody | Dilution | Band Size (kDa) |
|---|---|---|---|
Insulin receptor-β | Santa Cruz, sc-711 | 1:500 | >191 (IR precursor); 90 (IR-beta) |
IRS-1 | MilliporeSigma, 06-248 | 1:1000 | 180 |
Glut4 | Cell Signalling, 2213 | 1:1000 | 51 |
Adiponectin | Sigma, A6354 | 1:1000 | 30 |
ERK1/2 | Cell Signalling, 9102 | 1:1000 | 42, 44 (double band) |
P-Akt S473 | Cell Signalling, 9271 | 1:1000 | 60 |
P-Akt T308 | Santa Cruz, sc-16646-R | 1:1000 | 60 |
Akt | Cell Signalling, 4691 | 1:1000 | 60 |
α-Tubulin | Invitrogen, MA1-80017 | 1:1000 | 55 |
