Fig. 3: GBP1 targets Salmonella and controls recruitment of GBP2-4.

a Fluorescence confocal microscopy of naive or IFNγ-primed HeLa expressing N-terminal tagged eGFP-GBP1-7 (green) and infected with Salmonella-dsRed (red) for 1 h. DNA was stained with Hoechst (blue). Representative confocal images are shown and scale bars correspond to 10 µm. b Percentage of intracellular Salmonella positive for eGFP-GBP1-7 in naive or IFNγ-primed HeLa, at 1 h p.i. At least 200–300 bacteria were counted per coverslip. c Fluorescence confocal microscopy of IFNγ-primed wild-type or GBP1^–/– HeLa expressing eGFP-GBP2-4 (green) and infected with Salmonella-dsRed (red) for 1 h. Representative confocal images are shown, and scale bar corresponds to 10 µm. d Schematic representation of wild-type GBP1 and a ΔCaaX mutant. e, f Fluorescence confocal microscopy of naive GBP1^–/– HeLa expressing HA-GBP1^wt or HA-GBP1^ΔCaaX (e) or co-expressing mCherry-GBP1^wt and HA-GBP1^wt or HA-GBP1^ΔCaaX (f) and infected with Salmonella for 1 h. HA-tagged GBP1 was visualized by immunostaining with an anti-HA antibody. Representative confocal images are shown and scale bars correspond to 5 µm. The percentage of HA-GBP1 positive bacteria was quantified by counting around 100 bacteria per coverslip. Graphs show the mean ± SD, and data are pooled from two (b, e, f) independent experiments performed in duplicate or representative of two (a, c, e, f) independent experiments.