Fig. 2: Identifying a chimeric histidyl-tRNA.

a The cloverleaf structures of pylT and hisT with a G⁻¹ structure. The detailed sequence information of these tRNAs is shown in Supplementary Fig. 2. b The cloverleaf structures of chimeric histidyl-tRNAs (chHisTs) in which the sequence from pylT is coloured in green, while the sequence from hisT is coloured in blue and highlighted by a dashed box. The detailed sequence information of chHisTs is shown in Supplementary Fig. 2 as well. c Cartoon structure of chimeric histidyl-tRNA synthetase-1 (chHisRS-1) by fusion of pylRS TD (residues 1-149) and hisRS CD (residues 1-326). The truncation residue number is shown under the cartoon. d Amber suppression efficiency is tested by a GFP reporter assay. Wild-type GFP is expressed under the same condition as the control. The fluorescent intensity in each group is measured by a plate reader and normalised by the wild-type. Error bars represented ±standard error of the mean(n = 3). Source data are available in the Source Data file.