Fig. 6: Incorporation of Trp analogues with the chimeric PheRS/tRNA pair. | Nature Communications

Fig. 6: Incorporation of Trp analogues with the chimeric PheRS/tRNA pair.

From: Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion

Fig. 6

a Chemical structures of the Trp analogues used in this study. Analysis of amber suppression activity of indicated Trp analogues (2 mM) by chPheRS-3 (b) and chPheRS-4 (c) using GFP reporter assay in E. coli. The synthetic details of amino acids 6CNW and 7CNW are described in Supplementary Fig. 23. The chPheRS-3 is a 6CNW recognition synthetase, and the chPheRS-4 is a 7CNW recognition synthetase. Mass spectrometry characterisation of 6CNW (d) or 7CNW (e) incorporation in GFP. f HEK293T cells are co-transfected with two plasmids: one carrying chPheRS-4 gene and chPheT gene, the other bearing Histone H3-E106TAG gene with or without the addition of 2 mM of 7CNW. Transfected cells are then visualised by two-photon microscopy. Scale bar: 25 μm. Error bars represented ±standard deviation of the mean (n = 3). Source data are available in the Source Data file.

Back to article page