Fig. 3: Oligo copy distribution on the synthesis chip.

a The sequencing coverage of each oligo from the first ready-to-sequence pool was mapped back to its corresponding location on the synthesis chip. Coverages are normalized to the median. b The histogram of normalized sequencing coverage of the first ready-to-sequence pool (blue). The distribution does not fit a normal distribution (dashed line). c The sequencing coverage of each oligo from the second ready-to-sequence pool, mapped back to its corresponding location on the synthesis chip. Coverages are normalized to the median. d The histogram of normalized sequencing coverage of the second ready-to-sequence pool (blue). The second ready-to-sequence pool showed much more even oligo copy distribution and fits a normal distribution (dashed line) much more closely than then first. The unique number of sequences (sample size) is 1,536,168. Source data are available in the Source Data file.