Fig. 2: mutREAD reproducibly detects mutational signatures in FFPE samples.
From: The mutREAD method detects mutational signatures from low quantities of cancer DNA
a Comparison of the mutational signature profiles between WGS, fresh-frozen (FF) and FFPE samples for the same three EAC samples as in Fig. 1. Each bar indicates the contribution of the mutational signature (y-axis) to the overall mutational spectrum. Pairwise cosine similarities to WGS for the two mutREAD libraries are indicated above the bars. b Cosine similarity between mutational signatures derived from nine additional FFPE and WGS sample pairs and the number of detected mutations in the FFPE samples used to derive the mutational signatures. c Reproducibility of the sequenced regions between the first FFPE-derived technical replicate and the blood sample, the second FFPE-derived technical replicate and the blood sample, and between the two technical replicates (x-axis). The bars indicate the size of the overlapping regions in million base pairs (Mpbs, y-axis) for each comparison. Only regions covered at least 10× contribute to the comparison. The second technical replicate was sequenced to lower coverage and we down-sampled the first technical replicate by 50% to approximately match the sequencing coverage for comparison.
