Fig. 4: Representative image of the cell viability assay in HeLa MCTS.
From: Rationally designed ruthenium complexes for 1- and 2-photon photodynamic therapy
MCTS were treated with compounds 1–7 (20 μM, 2% DMSO, v%) in the dark for 3 days. After this time, MCTS were kept in the dark, exposed to 1P irradiation (500 nm, 16.7 min, 10.0 mW cm−2, and 10 J cm−2) or 2P irradiation (800 nm, 10 J cm−2, and section interval of 5 μm). After 2 days, the cell viability was assessed by measurement of the fluorescence of calcein (λex = 495 nm, λem = 515 nm), which is generated in living cells from Calcein AM. The scale bar represents a length of 200 µm.
