Fig. 6: Crb1-B is the most abundant Crb1 isoform in mouse and human retina. | Nature Communications

Fig. 6: Crb1-B is the most abundant Crb1 isoform in mouse and human retina.

From: Comprehensive identification of mRNA isoforms reveals the diversity of neural cell-surface molecules with roles in retinal development and disease

Fig. 6: Crb1-B is the most abundant Crb1 isoform in mouse and human retina.

a Retinal expression of top 3 Crb1 isoforms across mouse development, quantified from lrCaptureSeq dataset. A isoforms predominate at P1 but Crb1-B becomes most abundant by P6. Data were normalized to total Crb1 read counts at each timepoint (P1 = 923 reads, P6 = 6127 reads, P10 = 14,007 reads, Adult = 10,975 reads). b Expression of top 3 human CRB1 isoforms, quantified from adult human retina lrCaptureSeq dataset. c, d Short-read RNA-seq data was used to quantify top 3 mouse (c) or human (d) CRB1 isoforms. Mouse dataset (GSE101986; n = 2 biological replicates per time point) confirms developmental regulation of each isoform observed in PacBio data (a, c). Human dataset (GSE94437; n = 8 biological replicates) confirms CRB1-B is dominant isoform in adult macula (d, top) and peripheral retina (d, bottom). Lines (d) show measurements derived from same donor. FPKM, fragments per kilobase of transcript per million mapped reads. Statistics (d): One-way ANOVA with Tukey’s post-hoc test. ****P < 1 × 10−7. ***P = 1.6 × 10−6 (top); P = 6.6 × 10−6 (bottom). Error bars, 95% confidence interval of the FPKM value computed by Cufflinks software (c) or S.D. of the mean (d). For values, see Source Data file.

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