Fig. 1: Workflow of ribosome profiling. | Nature Communications

Fig. 1: Workflow of ribosome profiling.

From: MetaRibo-Seq measures translation in microbiomes

Fig. 1

a Experimental workflow of MetaRibo-Seq. Chloramphenicol halts translation, bacteria within the community are lysed, MNase is used to create footprints, and footprints are converted to sequencing libraries. b Computational workflow of the multi-omics approach. De novo assemblies are created and annotated, predicted genes are quantified at multi-omic levels, and taxonomy, correlations, and differential abundance are determined from these results.

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