Fig. 5: Odor tuning in the dorsal recess of the olfactory epithelium is similar to the dorsal olfactory bulb.
From: Antagonistic odor interactions in olfactory sensory neurons are widespread in freely breathing mice
a Left, experimental setup for imaging axon terminals of OSNs in the glomerular layer of the olfactory bulb from one of five mice used. Right, resting fluorescence of glomeruli and heatmaps of selected odor responses. Scale bar is 100 µm. b Left, experimental setup for imaging OSN somata in the olfactory epithelium from one of six mice. Right, resting fluorescence in the soma of GCaMP3-expressing OSNs in the olfactory epithelium and heatmaps of selected odor responses. Scale bar is 30 µm. c Odor tuning of 50 randomly selected glomeruli (left) and 50 OSN (right) across 32 monomolecular odors. Odor index is consistent for each panel and follows Table 3. d Fraction of glomeruli (left; n = 9 imaging fields) or somata (right; n = 6 imaging fields) that responded to each of the odors in a given imaging field. Grey lines are individual fields and dark line is the mean across all imaging fields. e Scatter plot of the responsive fraction across all glomeruli and somata for each of the 32 odors used. Dashed line is the linear regression to the data (Pearson’s correlation coefficient r = 0.750, P < 0.0001). f Odor tuning of 228 glomeruli (left) and 210 OSN somata (right). Grey lines are individual glomeruli or somata and dark line is the mean across all ROIs. g Scatter plot of the mean response across all glomeruli and somata for each of the 32 odors used. Dashed line is the linear regression to the data (Pearson’s correlation coefficient r = 0.598, P = 0.0003).
