Fig. 6: Antagonism in individual sensory neurons.
From: Antagonistic odor interactions in olfactory sensory neurons are widespread in freely breathing mice
a Experimental setup for imaging OSN somata in the epithelium. b Example image of OSN somata and selected ROIs from one of six mice. Odor responses for two odors (Methyl tiglate and Isobutyl propionate) and a mixture of both odors from selected OSNs are shown on right as ΔF/F time courses. Red bar under traces denotes odor delivery time. Scale bar in image is 30 µm. c Example dose response curves from two selected OSNs. Each point is the average of 3–5 trials. d Data from 20 randomly selected OSNs. Each point is the mean response of the three highest odor concentrations for each odor. Error bars are SEM. e Left, scatter plot of the observed mixture response against the linear sum of both mixture components or (right) against the maximum response generated by either mixture component. f Left, cumulative distribution of one-sided rank-sum P values obtained for 964 OSNs for linear sum comparison (black) and comparison to maximum component response (grey). Red dots mark P values < 0.05. Right, cumulative distribution of left-sided rank-sum P values for linear sum comparison (black) and comparison to maximum component response (grey) to identify synergistic mixture interactions.
