Fig. 3: TAMs frequently neighbor tumor cells near vasculature and fibrillar extracellular matrix. | Nature Communications

Fig. 3: TAMs frequently neighbor tumor cells near vasculature and fibrillar extracellular matrix.

From: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells

Fig. 3: TAMs frequently neighbor tumor cells near vasculature and fibrillar extracellular matrix.The alt text for this image may have been generated using AI.

a, b Representative IVM of HT1080 tumors grown subcutaneously in the dorsal window chamber of MertkGFP/+ NOD.SCID mice at low (left) and high (right) magnifications. a A long-circulating fluorescent NP, angiospark-680, and b 2-photon microscopy of second harmonic generation, SHG show tumor vasculature and fibrillar extracellular matrix, respectively. At bottom right, EB3 tracks are shown randomly pseudo-colored for visualization. Scale bars are 1 mm (a, right), 50 μm (a, b, left), and 10 μm (b, right). c Corresponding to images as in (a), tumor cells were scored based on nearest proximity (<5 μm) to GFP+ TAMs or AngioSPARK+ vasculature (data are means ± s.e.m.). For all, n = 80 total cells and n = 4 tumors overlaid as individual data points in (c). Source data are provided as a source data file.

Back to article page