Fig. 4: IL4-polarized MΦ promote coherent MT alignment in neighboring tumor cells. | Nature Communications

Fig. 4: IL4-polarized MΦ promote coherent MT alignment in neighboring tumor cells.

From: In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells

Fig. 4: IL4-polarized MΦ promote coherent MT alignment in neighboring tumor cells.The alternative text for this image may have been generated using AI.

a, b Representative fluorescence microscopy after 24 h of IL4-MΦ co-culture with EB3 tracks randomly pseudo-colored for visualization (scale bar = 10 μm). c Corresponding to a, b, the effect size for imaged MT features was compared between monoculture and 24 h MΦ co-culture (average of Cohen’s D effect size between batches). d From c, track distributions for the top two increased MT features, altered in both cell lines with co-culture, are shown with cell averages overlaid as individual data points (bar denotes median). All p values were computed using a two-tailed permutation test with BH correction. For HT1080 cells, n = 22,371 tracks across n = 164 total cells were analyzed, and for ES2 cells n = 1424 tracks across n = 33 total cells were analyzed. Source data are provided as a source data file.

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