Fig. 1: Liver organoid growth in Matrigel and PEG supplemented with ECM components.
From: Mechano-modulatory synthetic niches for liver organoid derivation
a Mouse liver progenitor cells 3 days after embedding in: Matrigel (MG), plain PEG (PEG) and PEG functionalized with the indicated ECM components: COL.IV (collagen IV), FN (fibronectin), LAM-1 (laminin-1) and RGD-representing peptide (RGD). b Quantification of organoid formation efficiency relative to panel a. Graphs show individual data points derived from n = 3 independent experiments and means ± s.d., one-way Anova. *P < 0.05, ***P < 0.001. P = 0.0321; P = 0.0266; P = 0.0119; P = 0.0003. Source data are provided as a Source Data file. c Hematoxylin and eosin staining of Matrigel- and PEG-derived organoids. Scale bar 25 μm. d Gene expression was analysed by qRT-PCR in liver organoids 6 days after embedding in Matrigel and PEG hydrogels supplemented with different ECM factors. The heatmap represents ΔCt values as described in the method section. e Liver organoid immunostaining was performed 6 days after embedding of liver progenitor cells in Matrigel or PEG-RGD. f Liver organoids can be cultured in PEG hydrogels by directly embedding mouse biliary duct fragments. Representative pictures are shown. Micrographs (c, e, f) are representative of three independent experiments.
