Fig. 2: Increased glutamine is sufficient to inhibit melanoma growth.

a Nude mice with M229 xenografts received control, amino acids, or high Gln diet. Tumours were measured twice weekly (Control, n = 10; Amino acids, n = 6; High Gln, n = 7). b Tumour weights post mortem (Control, n = 10; Amino acids, n = 6; High Gln, n = 7 biologically independent tumours). c Glutamine concentration in serum measured by EIA Kit (Control, n = 4; Amino acids, n = 5; High Gln, n = 4 biological replicates performed in triplicates). d, e Nude mice with subcutaneous injection of M229 cells receiving control, amino acids, or High Gln diet were weighed in d and monitored for food intake in e (Control, n = 10; Amino acids, n = 6; High Gln, n = 7). f Nude mice with subcutaneous injection of M229 cells placed on regular (control) water or glutamine-supplemented (Gln) water 1 week post injection. Tumours were measured twice weekly (Control, n = 7; High Gln, n = 7). g Tumour weights post mortem (Control, n = 7; High Gln, n = 7 biologically independent tumours). h Glutamine concentration in serum was measured (Control, n = 7; High Gln, n = 7 biological replicates performed in triplicates). i Nude mice with subcutaneous injection of M229 cells placed on control water or Gln water were weighed twice weekly (Control, n = 7; High Gln, n = 7 biologically independent mice). Data represent means and error bars are s.d. a–e p value calculated using one-way ANOVA followed by post hoc Tukey’s HSD test. f–i p value calculated by t test (unpaired, two tailed), *p < 0.05, **p < 0.01, n.s. not significant.