Fig. 3: High glutamine impedes growth independent of BRAF status.

a, b NSG mice with subcutaneous implantation of melanoma PDX tumour TM00702 (Control, n = 10; High Gln, n = 9 biologically independent tumours) received control or high glutamine (High Gln) diet and tumours were measured twice weekly (a) and tumour weights post mortem are shown in b. c, d NSG mice with subcutaneous implantation of melanoma PDX tumour TM01612 (Control, n = 8; High Gln, n = 8 biologically independent tumours) received control or High Gln diet and tumours were measured twice weekly (c) and tumour weights post mortem are shown in d. e, f Glutamine concentration in PDX TM00702 in e and TM01612 in f from serum and tumours were measured (n = 8 biological replicates). g Cross-sections of PDX_TM00702 (left) and PDX_TM01612 (right) tumours were H&E stained. Scale bar = 100 μm. Arrows indicate regions of necrosis/apoptosis. h Cross-sections of PDX_TM00702 (left) and PDX_TM01612 (right) tumours were Ki67 stained (Control, n = 3; High Gln, n = 3). Scale bar = 100 μm. Data represent means and error bars are s.d. p value was calculated by t test (unpaired, two tailed) except in a, c by Mann–Whitney test (unpaired, two tailed). *p < 0.05, **p < 0.01.