Fig. 6: κB-Ras deficiency promotes acinar-to-ductal metaplasia and Sox9 expression. | Nature Communications

Fig. 6: κB-Ras deficiency promotes acinar-to-ductal metaplasia and Sox9 expression.

From: κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis

Fig. 6

a Resected tumours of 10-week-old 1SKO/R and pDKO/R mice (left) and exemplary haematoxylin/eosin staining of a section of 10-week-old pDKO/R mice (right). Scale bar: 500 µm. b Fluorescent IHC staining of CK19 (magenta) and amylase1 (green) in pancreatic sections of 1SKO/R and pDKO/R littermate mice (4 weeks old). Scale bar: 200 µm. c FACS analysis of pancreatic cell populations. Acinar cells were excluded from analysis based on FSC/SSC and residual cells analysed for ductal (MIC-1C3+) and acinar cell markers (MPx1+) by surface staining. d Fluorescent IHC staining of Cpa1 (magenta, acinar cell marker), Sox9 (green) and nuclei (DAPI, blue) in paraffin sections of 1SKO/R and pDKO/R littermate mice (4 weeks old). Scale bar: 200 µm.

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