Fig. 1: Sample preparation methods for cryo-EM.
From: Modular microfluidics enables kinetic insight from time-resolved cryo-EM
a Drawing of the standard method, which entails manually pipetting several microliters of sample onto a support grid, removing the excess liquid by blotting (touching with filter paper) and vitrification by plunge-freezing into liquid ethane. The most popular commercial system for this preparation is marketed as Vitrobot, and this name is used interchangeably with standard cryo-EM sample preparation throughout the text. b Drawing of time-resolved cryo-EM sample preparation. A microfluidic device mixes and incubates a biochemical reaction, then sprays the sample onto a plunging grid such that blotting is not required, and finally the sample grid is plunged into liquid ethane to achive vitrification.
