Fig. 3: Sample spray and blot-free delivery onto a TEM support grid.
From: Modular microfluidics enables kinetic insight from time-resolved cryo-EM
a Schematic representation of a microfluidic device with a gas-assisted spraying nozzle. b Snapshots from high-speed camera datasets of sample sprayed at indicated nominal gas pressure. Scale bar is 363 μm. c, d Quantification of droplet diameters (c) and speed (d) as a function of increased gas pressure. Error bars represent standard deviation across the total population of droplets quantified. Source data are provided as a Source Data file. e, f Snapshots from high-speed camera imaging of a cryo-EM sample grid plunging (e) past the nozzle (nozzle position indicated in dotted green) and (f) from the nozzle into the liquid ethane container (positions indicated by white arrows), with time stamps in milliseconds.
