Table 1 Cryo-EM data collection and refinement statistics.
From: Modular microfluidics enables kinetic insight from time-resolved cryo-EM
Vitrobot apoferritin (EMDB-10714) | Sprayed apoferritin (EMDB-10712) | |
|---|---|---|
Data collection and processing | ||
Magnification | 96,000 | 96,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e–/Å2) | 33.6 | 33.6 |
Defocus range (μm) | −0.5 to −2.5 | −0.5 to −3.5 |
Pixel size (Å) | 0.845 | 0.845 |
Symmetry imposed | o | o |
Initial particle images (no.) | 146,581 | 133,833 |
Final particle images (no.) | 19,485 | 9804 |
Map resolution (Å) | 2.89 | 2.77 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 2.84–3.15 | 2.82–3.19 |
2D classification | 99,271 particles after two rounds of 2D classification | 65,202 particles after three rounds of 2D classification |
3D classification | 19,485 particles after one round of 3D classification | 9804 particles after one round of 3D classification |
