Fig. 4: The INTS10–INTS13–INTS14 complex binds nucleic acids with a preference for stem loop RNAs. | Nature Communications

Fig. 4: The INTS10–INTS13–INTS14 complex binds nucleic acids with a preference for stem loop RNAs.

From: INTS10–INTS13–INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module

Fig. 4: The INTS10–INTS13–INTS14 complex binds nucleic acids with a preference for stem loop RNAs.

a–d EMSAs of INTS13–INTS14 (top panels) and INTS10–INTS13–INTS14 (lower panels) with U1 3′-box ssRNA a, U1-SL4 dsRNA b, U1 3′-box ssDNA c, and dsDNA d. Increasing protein concentrations were incubated with the respective nucleic acid, resolved on a native acrylamide gel and stained with a fluorophore. e Fluorescence polarization of FAM-labeled U1 3′-box ssRNA with INTS13–INTS14 (purple) and INTS10–INTS13–INTS14 (black), respectively. Error bars indicate standard deviations from the mean of triplicate experiments. f Dissociation constants with standard error of means derived from fitting data in e. Source data are provided as a Source Data file.

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